智慧城市網
資料下載
恒遠產品文獻:魚蛋白酶（Protease）ELISA試劑盒引用文獻
閱讀：488 發布時間：2022-11-29| 提 供 商 | 上海恒遠生物科技有限公司 | 資料大小 | 5.3MB |
|---|---|---|---|
| 資料圖片 | 下載次數 | 19次 | |
| 資料類型 | PDF 文件 | 瀏覽次數 | 488次 |
| 免費下載 | 點擊下載 |
【文獻標題】High protein intake promotes the adaptation to chronic hypoxia in zebrafish (Danio rerio)
【作者】Qiang Ma , Xue Wang , Ling-Yu Li , et.al
【作者單位】華東師范大學（East China Normal University）
【文獻中引用產品】
魚蛋白酶（Protease）ELISA試劑盒
【關鍵詞】Chronic hypoxia ,High protein intake ,Metabolism, Microbiota ,Zebrafish
【DOI】doi.org/10.1016/j.aquaculture.2021.736356
【影響因子(IF)】5.4
【出版期刊】《Aquaculture》
【產品原文引用】
2.4. Diets and body composition analyses and biochemical assays
Moisture of diets and whole fish was measured after dry at 105 ?C with 24 h. The total protein content of diets and whole fish were measured by a semi-automatic Kjeldahl System (FOSS, Sweden) after sulfuric acid digestion. The total lipid of diets and whole fish were extracted using chloroform/methanol (2:1, v/v) (Lambert and Dehnel, 1974). The activities of lactate dehydrogenase (LDH), glutamic propylic transaminase (GPT), glutamic oxaolacetic transaminase (GOT), lipase, amylase, and the contents of glycogen, lactate, hemoglobin, triglyceride (TG), total cholesterol, total amino acid (TAA) and the protein (BCA method) in tissues were determined using commercial kits (Nanjing Jiancheng Bioengineering Institude, China). Protease activity of gut was determined using fish Protease enzyme-linked immune sorbent assay (ELISA) kit (Shanghai Hengyuan Biological Technology Co., LTD, China). All measurement steps refer to relevant kit protocol and the results were recorded on a microplate reader (Epoch, BioTek, USA). Glucose content of blood was detected by using a blood glucose monitor (Glucose dehydrogenase method, CONTOUR?PLUS Blood Glucose Monitoring Systems, Bayer).
完整版PDF文獻請咨詢在線客服或者聯系我司業務員！
更多公司福利請關注“恒遠生物"微信公眾號！